Flow cytometric analysis of activated BM-derived DCs.

(A) FL-supplemented BM cells from C57BL/6 mice were cultured for 9 days and stimulated with LPS, IFN-α, or GM-CSF during the last 24 hours of culture. (B) Three-color analysis of FL-derived (± LPS) DCs with CD11b and CD11c used to define myeloid- and lymphoid-type DCs. (C) DCs generated in BM cultures supplemented with GM-CSF and IL-4 for 7 days. Gates were determined with the use of isotype controls as described in “Materials and methods.” Results shown are representative of more than 5 experiments.