Comparison of allo-stimulatory activity of FL-derived and GM-CSF plus IL-4–derived DCs.
DCs were enriched from spleens of mice treated with FL and generated from the BM of C57BL/6 mice cultured in GM-CSF plus IL-4 or in FL, as described in “Materials and methods.” Splenic-derived DCs from mice treated with FL for 10 days (■) and BM-derived DCs from cultures using GM-CSF and IL-4 (●), FL alone (▴), FL plus IFN-α (♦ and dashes), or FL plus LPS (▪) were cultured at various numbers in the presence of a constant number (1 × 105 per well) of T cells from DBA/2 mice for 4 days. Alamar blue was added for another 24 hours before measuring optical density (OD). DBA/2 T cells alone gave a mean OD of −0.044 ± 0.031. Background OD from DCs alone was subtracted from DCs with T cells.
