Comparison of antigen processing and presentation by DCs generated from FL-supplemented or GM-CSF plus IL-4–supplemented BM cultures.
(A) Splenic-derived DCs from mice treated with FL for 10 days (▪) and BM-derived DCs from cultures using GM-CSF and IL-4 (●), FL alone (▴ and dashes), or FL plus LPS (♦) were cultured with constant OVA protein (300 μg/mL) and 1 × 105 cells per well OVA-specific D011.10 T cells. (B) DC and T cells were cultured at constant concentrations (2 × 104 DCs per well), and OVA protein was titrated. T-cell proliferation was measured on day 5 for both assays. Background counts from OVA-specific T cells cultured without DCs in OVA protein (300 μg/mL) were fewer than 4000 cpm, and T cells without OVA protein and with DCs (2 × 104 DCs per well) were fewer than 1000 cpm. Background counts from DCs alone were fewer than 1000 cpm. Data represented are the mean ± SD of triplicate wells, and the experiement was performed 5 times.
