Fig. 2.
Effect of IFN-γ and IL-6 on the susceptibility of macrophages to HIV-1NL4-3 entry.
(A) Six-day MDMs untreated or treated with IFN-γ (for the last 24 hours) or with IL-6 (for the last 48 hours) were infected with NL4-3 for 48 hours. DNA was extracted from infected and uninfected MDMs and amplified by PCR using gag-specific primers. (B) DNA from serial dilutions of ACH-2 cells (containing one proviral copy/cell) was amplified by PCR as an internal standard control. Data represent 3 experiments.