Fig. 2.
Relative sensitivities to PEG-rHu MGDF of circulating megakaryocytic progenitor cells from patients with ET and healthy subjects, determined in strictly serum-free culture.
Cells from 11 ET patients (▴) and 11 healthy subjects (▪) were simultaneously compared with respect to numbers of megakaryocytic colonies produced as a function of PEG-rHu MGDF concentration and the data were normalized as percentage of maximum colony number (mean ± SEM ); see Table 2 for details. The first 6 samples were performed in quadruplicate mini-macro wells at 1.5 × 105 cells per 0.7 mL medium; the last 5 were performed in quintuplicate microwells at 4 × 104 cells per 0.1 mL medium.
