Flow cytometric analysis of TF expression in fibroblasts.

(A) Cells were stained with either a murine monoclonal FITC-conjugated mouse anti-IgG antibody (unfilled area) that was used as a negative control or a monoclonal FITC-conjugated anti-tissue factor (TF) antibody (filled area). Serum-starved cells expressed almost the same levels of TF (data not shown). (B) Procoagulant activity of fibroblasts. Fibroblasts with TF expression generated a 10-fold increase in PCA compared to monocytes without TF expression.