DMT1 mRNA expression in intestine from anemicmk/mk mice.

The proximal portion of the small intestine immediately adjacent to the stomach was dissected from mk/+ heterozygotes andmk/mk homozygous anemic mice (2 animals in each group). Total RNA from each tissue was prepared, separated by electrophoresis through a denaturing agarose gel, and followed by blotting to a hybridization membrane. The blot was probed with a DMT1 cDNA probe that recognizes both the IRE (isoform I) and non-IRE (isoform II) containing mRNAs (A) and was washed under highly stringent conditions. After removal of the probe, the blot was sequentially rehybridized with probes for TfR (B) and β-actin (C).