Fig. 4.
![Fig. 4. Only mature monocyte-derived DCs can initiate a CD4+ T-cell response to a neoAg in a primary proliferation assay. / Immature DCs (top) or mature DCs (bottom) were pulsed on day 3 of culture with TT as a recall Ag or KLH as a neoAg, and were plated at day 8 with autologous CD4+ T cells at various T/APC ratios. T-cell Ag-specific proliferation was measured by [3H]-thymidine incorporation at day 6 after coculture initiation for TT, or at day 7 for KLH. Unpulsed DCs were used as negative controls for the 6- and 7-day assays, and these controls are shown separately. Each point was done in triplicate. These results are representative of 3 experiments with 3 different donors.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/10/10.1182_blood.v96.10.3490/5/h82100326004.jpeg?Expires=1735819567&Signature=hhv1rUbsegmHZOd-TL4SgkAV88pKCWNCjGVraj2Uw3qbq2zuN2OLB1Yt9AL59XOVM2lqPICxhzL1zgNnNPSw2XUJBop7uNBRUm-3L6ITGamW2Mh77GbfU7zUUrUpYyE21HQhdpbdhWwS-Adijebfzz1rC8HA5j1nnCoNX-jxdLJEpFotQUB6OiJDaiNnMX8Nq7h9KUdrTOgUZWpg3mNsFyqMT6R9G0uNHpdgARP8W5JmfA6PBZGt4XC14Fiw9u8BrrkDy~5-XB4Fr8frJh4nbLX~ds48ZmIkZupsDkP83euLd7lWEVaxf3cp8XBerob1CG-RGUdytgc6b92pacdihQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Only mature monocyte-derived DCs can initiate a CD4+ T-cell response to a neoAg in a primary proliferation assay.
Immature DCs (top) or mature DCs (bottom) were pulsed on day 3 of culture with TT as a recall Ag or KLH as a neoAg, and were plated at day 8 with autologous CD4+ T cells at various T/APC ratios. T-cell Ag-specific proliferation was measured by [3H]-thymidine incorporation at day 6 after coculture initiation for TT, or at day 7 for KLH. Unpulsed DCs were used as negative controls for the 6- and 7-day assays, and these controls are shown separately. Each point was done in triplicate. These results are representative of 3 experiments with 3 different donors.
