Fig. 1.
Effects of Cd at the indicated doses in Hep3B cells with or without exposure to hypoxia (1% O2) or Co (100 μM).
(A) EPO protein production and cell viability at 24 hours. (B) Cell viability at 6 hours. (C) EPO mRNA expression at 6 hours. (D) DNA binding activity of hypoxia inducible factor-1 (HIF-1) at 6 hours. In C, RNase protection revealed 2 bands of protected EPO mRNA (75 bp and 61 bp). In D, the specificity of HIF-1 bands (H) and constitutive bands (C) was confirmed by the addition of excess unlabeled W18 oligonucleotide as a competitor for HIF-1 binding. N indicates nonspecific band. Data in A and B are expressed as mean ± SD of 3 independent experiments. Results in C and D are representative of 3 independent experiments.
