Subunit polypeptides of the patient's fibrinogen examined by SDS-PAGE.

(A,B) Lane 1, normal fibrinogen; lane 2, the patient's fibrinogen. The bands representing 2 molecular weight species of the patient's fibrinogen indicated by Fbg and Fbg′ (lane 2 in panel A) in 4.5% gel were cut out, and SDS-PAGE was rerun on a 7.5% to 15% gradient gel under nonreducing (C) and reducing conditions (D). (C,D) Lane 1, normal fibrinogen; lane 2, the higher molecular weight species; lane 3, the normal fibrinogen species. Note that only an aberrant Bβ chain (Bβ′) is present in the Fbg′ species.