Compaction studies of the patient's fibrinogen.

The fibrin clot was formed in a conical microfuge tube with 0.60 mg/mL fibrinogen and 0.06 NIH U/mL thrombin in Tris-buffered saline. Following incubation at 25°C for 2 hours, the tubes were centrifuged at 4200g for 30 seconds 3 times, and each volume of the expelled buffer was withdrawn and measured with a Hamilton syringe. Percent compaction was expressed as the volume percent of the original volume (0.75 mL) of the clot.