Both lentivirus and MLV vectors transduce stem cells.

Southern blot analysis of DNA extracted from the BM (M), spleen (S), and thymus (T) of animals was transplanted with cells transduced for 96 hours in cytokines with M-GFP (MLV group) and L-GFP (lenti group). DNA was collected 16 weeks after transplant and digested withSst1, which cuts twice in each vector, producing a 2.3-kb fragment for M-GFP and a 5.4-kb fragment for L-GFP, andNco1, which cuts once in each vector, for insertion-site analysis. The membranes were probed with a P32-labeled GFP probe.