Fig. 2.
Induction of cyclin A1 by expression of PLZF-RARα.
(A) PLZF-RARα was induced by ZnSO4 (0.1 mmol/L, 24 hours) in U937-B412 cells. The immunoblot was performed as described in the legend for Figure 1. (B) Cyclin A1 RNA levels before and after induction of PLZF-RARα in B412 cells (Northern blot, upper panel). The same blot was also probed with a cyclin A probe after stripping of the cyclin A1 probe (middle panel), and no difference was observed for cyclin A. Equal loading was shown by staining for the 18s RNA. B412 cells were induced to express PLZF-RARα for 24 hours, and total RNA was isolated. On each lane, 20 μg RNA was loaded.
