Fig. 4.
ATRA reduced levels of cyclin A1 RNA in the APL cell lines UF-1 and NB4.
(A) Northern blots showing the time course and dose response of cyclin A1 mRNA levels in UF-1 cells exposed to ATRA. In the time-course study, 10−6 mol/L ATRA was used. In the dose-response study, cells were harvested 24 hours after treatment. On each lane, 20 μg total RNA was loaded, and equal loading was confirmed by probing the blot with an actin probe. (B) NB4 cells were studied in a manner similar to that described in panel A.
