Fig. 8.
Activation of the cyclin A1 promoter by PML-RARα and reversal of this effect by ATRA.
U937 cells were transiently transfected with cyclin A1 promoter-luciferase constructs and either PML-RARα expression vector or an empty vector control. Expression of a renilla luciferase expression vector was used for standardization purposes. (A) Activity of the empty luciferase reporter vector PGL3-Basic was reduced by 80% upon PML-RARα expression. In contrast, the cyclin A1 promoter constructs were activated more than 2-fold (1344-bp construct) or 1.7-fold (335-bp construct). (B) The activating effects of PML-RARα were reversed when ATRA (10−6 mol/L) was added after electroporation.
