Fig. 3.
Endothelial [Ca2+]i elevations evoked by a suspension or supernatant of PMNs.
When a concentrated PMN suspension (3 × 106 cells/mL; A) or its cell-free supernatant (B) was perfused through the EC monolayer, EC [Ca2+]i elevations were clearly visible in either case. In contrast, the supernatant from a diluted PMN suspension (5 × 105 cells/mL) induced little EC [Ca2+]i elevation (C). The arrows mark the time at which the PMN suspension or supernatant reached the EC monolayer.