Fig. 5.
Elevation of endothelial [Ca2+]i at an early stage of PMN transmigration.
A PMN arrives at 375 seconds (arrow). The transmigration-associated EC [Ca2+]i elevation happens at about 430 seconds, and the fixative arrives shortly afterward at about 450 seconds. The top panel shows confocal images of the fixed specimen. The positions of these optical sections relative to the apical surface of the EC monolayer (0 μm) are labeled underneath each image. Bar equals 20 μm.