Fig. 6.
![Fig. 6. Blockage of PMN transmigration by [Ca2+]i-buffered ECs. / ECs were pretreated with 50 μmol/L BAPTA-AM for 25 minutes. Both phase-contrast dynamic images (top panel) and confocal optic sections at the end (bottom panel) are shown. When EC [Ca2+]i elevation is prevented with BAPTA treatment (curve not shown), the PMN forms protrusions that extend underneath the EC monolayer. Although the protrusions repeatedly change shape and direction, the cell body is unable to pass through the monolayer. Bars equal 20 μm.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/12/10.1182_blood.v96.12.3816/5/h82300408006.jpeg?Expires=1735872953&Signature=4~APcxBZ5YrZ6hCLeGczqTdB1~X4x5xeJhq8~dhCEEM1wc5yX5QoPcWt713WyuTu7xz5ZaLWdUn5TGx8y~ZVLHldp6ShjVusK-qQLIgy75X1pCBPqfP8r17tzrB~5dApIjQM8XPR6ywnS6Sz6afrnVP5h4kN9brxhQUV8fQomMQC8JjGKiC0smfm0BooWdGf63h0CpXq3GVG9IBjOeHpy50q4UNmEdF4nALiSrcMzdf5-8Mn1TKGhUC9fJbklIr9PeHNUzIXBjwJpfMHaZ9z5qRhdNZGSK-U3tJha8V5h8P6WjuRvKh1LNok81yJbr2kXl~QfOW3aPxbRKY6WFgsBQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Blockage of PMN transmigration by [Ca2+]i-buffered ECs.
ECs were pretreated with 50 μmol/L BAPTA-AM for 25 minutes. Both phase-contrast dynamic images (top panel) and confocal optic sections at the end (bottom panel) are shown. When EC [Ca2+]i elevation is prevented with BAPTA treatment (curve not shown), the PMN forms protrusions that extend underneath the EC monolayer. Although the protrusions repeatedly change shape and direction, the cell body is unable to pass through the monolayer. Bars equal 20 μm.
