Fig. 7.
![Fig. 7. Transmigration of [Ca2+]i-buffered PMN and concomitant EC [Ca2+]i elevation. / The PMN was pretreated with 50 μmol/L BAPTA-AM for 25 minutes. The transmigration process appears normal at the early stages (top panel, up to 410 seconds), but the transmigrated PMN becomes elongated at the later stages (top panel, 910 seconds). The confocal microscopy images (middle panel) show a 3-dimensional view of this elongated PMN that was fixed after the last phase-contrast image was taken (star indicates rear end; arrow indicates front end). Bars equal 20 μm. The bottom panel shows the [Ca2+]i elevation in ECs adjacent to the PMN transmigration site. This PMN arrives at 220 seconds.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/12/10.1182_blood.v96.12.3816/5/h82300408007.jpeg?Expires=1735872805&Signature=Z5wBSBBJWk-8S8obbWkIwZXaltTQxYEjFHhKeQKQGGp5DCjHi9UWwujMP6zywMCd3ifgdYsa~SfjwuWHyhWjDdWBGVe2vUhyV6Uj6NBv6JR0gDp-3D9cQA~zpsWGMHdwnPo84pQfMZtZROlUYSFCTK22mNRA8C9W7yEFIf~Tw354pB3MY1PqBBblZNwK1t1K~M8NO1wT-l39o2uY3wRqqWZoQhEQf9lq1G61ERKaocIaUix~hoot9ic02rZrqsPlig461CXXJ84neJ5o1XN0yY1nqBa4bfYSpsddblLXRd6Q9nsSe-82ZDMpg2zRaC9ie-6chjiK5AnW1HMco5lbSQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Transmigration of [Ca2+]i-buffered PMN and concomitant EC [Ca2+]i elevation.
The PMN was pretreated with 50 μmol/L BAPTA-AM for 25 minutes. The transmigration process appears normal at the early stages (top panel, up to 410 seconds), but the transmigrated PMN becomes elongated at the later stages (top panel, 910 seconds). The confocal microscopy images (middle panel) show a 3-dimensional view of this elongated PMN that was fixed after the last phase-contrast image was taken (star indicates rear end; arrow indicates front end). Bars equal 20 μm. The bottom panel shows the [Ca2+]i elevation in ECs adjacent to the PMN transmigration site. This PMN arrives at 220 seconds.
