Appearance of 5-LO and FLAP proteins during DC differentiation of HPCs.

HPCs were maintained in culture as described in Figure 1 in medium supplemented with standard medium (S) or in the presence of standard medium plus TGF-β–1 (S + T) for 11 days. Immunoblots were performed on 10 μg total cell protein using anti–5-LO antiserum, LO-32, or a polyclonal anti-FLAP antiserum. To obtain sufficient protein from freshly isolated HPCs at day (d) 0, pools of 3 separate CB preparations were used. Arrows at right represent the positions of molecular weight standards; asterisks indicate the position of (A) recombinant 5-LO standard or (B) leukocyte membrane protein for FLAP.