Heterogeneity of 5-LO⁺ leukocytes derived from CD34⁺ HPCs.

HPCs were maintained in culture for 15 days as described in Figure 2 in standard medium (panels A-D, F, I, and J) or standard medium plus TGF-β–1 (panels E, G, and H). Cytospins were stained with antisera as described in “Materials and methods”; 5-LO throughout Cy3 red, marker antisera throughout Cy2 green. (A) HLA-DR/5-LO, identical phase contrast field but stained with (B) DNA stain Hoechst 33258. Upper arrow indicates an HLA-DR⁺/5-LO⁻ cell; lower arrow indicates an immature HLA-DR⁻/5-LO⁻cell. (C) CD1a/5-LO and identical phase contrast field but stained with (D) DNA stain Hoechst 33258, (E) CD40/5-LO, (F) CD83/5-LO, (G) Lag antigen/5-LO, (H) eosinophil peroxidase/5-LO, (I) CD14/5-LO, and (J) mannose receptor/5-LO. Bars indicate 20 μm.