Inhibition of factor IXa binding to LRP by heparins.

Binding of 100 nmol/L of factor IXa to immobilized LRP (26 fmol/mm²) was analyzed by SPR in the absence or presence of heparin (●) or fragmin (■) (0-100 U/mL) or 100-μg/mL chondroitin sulfate (▴). Binding is expressed as the percentage of binding in the absence of competitor and is corrected for nonspecific binding (< 5%). Data represent the mean ± SD of 3 experiments.