Fig. 5.
Role of LRP and proteoglycans in factor IXa degradation by CHO cells.
Degradation was assessed by incubating wild-type (WT, black bar), LRP-deficient, (LRP-def, white bar), and proteoglycan-deficient (PG-def, gray bar) CHO cells with 125I-radiolabeled factor IXa (40 nmol/L) for 30 minutes at 37°C. After washing, bound material was incubated for an additional 5 hours, and degradation was determined as described in “Materials and methods.” Degradation of factor IXa by wild-type CHO cells is referred to as 100% and corresponds to 0.12 pmol/2.5 × 105 cells after 5 hours of incubation. The data represent the mean ± SEM of 3 experiments.
