The survival of biotin-labeled HDE cells.

For 3 patients with sickle cell disease, a high-density fraction was isolated, labeled with biotin, and re-infused. The average number of labeled cells present in blood samples collected between 7.5 and 20 minutes after re-infusion was taken as 100%. The top panel also shows the expected survival of normal RBCs and the range of survival for unfractionated sickle cells from Franco et al.10