Absence of lamellae in human platelets preincubated with wortmannin or in gelsolin-deficient mouse platelets.

Platelets stimulated as in Figure 1 were examined for shape change by video microscopy. (A) Effect of 50 nmol/L wortmannin preincubated for 15 minutes on the shape change of human platelets exposed to a CRP-coated surface. (Inset) Shape change of human platelet on CRP-coated surface in the absence of wortmannin. (B) Gelsolin-deficient mouse platelets activated on a CRP-coated surface. (Inset) Mouse WT platelets on a CRP-coated surface. Images were taken after 15 minutes of activation. Results shown are representative of 3 experiments.