IL-1β inhibits IL-6-induced luciferase gene expression driven by rat γ fibrinogen promoter.

(A) Constructs F2 and F3 were transfected into human hepatoma HepG2 cells. Cytokine (IL-1β and IL-6) treatment is indicated. Results presented are means of fold induction above the control level from triplicate experiments. (B) Overexpression of IL-6 receptor does not affect the IL-1β inhibition. The reporter construct F3 was co-transfected with pCMV-RIL6R into human hepatoma HepG2 cells. After transfection, the cells were treated with recombinant mouse IL-6 or IL-1β as indicated. The results presented are means of fold induction over the control levels from triplicate experiments. The error bars in A and B indicate standard deviation.