IL-1β-activated protein complexes associate with the site II and site III probes.

EMSA was performed as described in “Materials and methods.” Nuclear extracts were prepared from rat primary hepatocytes following exposure to the indicated cytokines. Site I, site II, and site III probes are derived from the 3 IL-6REs of the rat γ fibrinogen gene. The arrows on the right side indicate the migration positions of 2 different protein complexes, the IL-6-induced STAT3 complex, and the IL-1-induced complex X.