Characterization of cord blood CD34⁺ HPC populations according to CD7 and CD45RA expression.

Purified CD34⁺ cells were labeled with CD34-PECy5, CD7-FITC, and CD45RA-PE mAbs and analyzed by 3-color FACS analysis. Cells were gated based on low forward and side scatters and high CD34 expression (R1). Sort windows R2, R3, and R4 were set based on labeling with control mAbs (data not shown). CD7⁺CD45RA⁻, CD7⁺CD45RA⁺, and CD7⁻CD45RA⁺CD34⁺ HPC percentages are means ± SD (n = 17).