Expansion and differentiation into DCs of SP (CD7⁻45RA⁺), DP (CD7⁺45RA⁺), and bulk CD34⁺ HPCs cultured under the standard condition.

(A) Viable cell numbers were normalized relative to 1 × 10⁵ seeded HPCs; means ± SD (n = 5 to 8); differences were significant on days 5, 8, and 12 (P =  .03 to .007) between DP and SP HPCs, and on day 8 (P =  .001) and 12 (P =  .0005) between DP and bulk HPCs. (B) FACS analysis of day 8 CD1a-FITC-labeled cells; means ± SD CD1a⁺ DC percentages (n = 8); differences between DP and SP or bulk HPCs (P ≤ .001), between SP and bulk HPCs (P = .07).