Levels of huCD64 expression on PMN.

HuCD64⁺ mice were treated with murine G-CSF, 2 μg/d, subcutaneously, for 4 days. Levels of huCD64 on PMN were determined by FACS analysis of whole blood incubated with FITC-labeled M22 (anti-huCD64). The comparative expression levels on nontransgenic (A), unprimed (B), and primed (C) transgenic mice are shown. The level of staining given by a control irrelevant FITC-labeled IgG on PMN from Tg and nonTg was the same as that shown by curve A. The inset shows the scatter profiles, with the percentages of PMN (gated) in the total leukocyte population before and after the course of G-CSF.