Fig. 9.
![Fig. 9. Measurement of the levels of intracellular free calcium in lymphoma cells treated with BsAb. / πBCL1 were loaded with Indo-1-am fluorescent dye, warmed to 37°C for 5 minutes, and then incubated with either BsAb alone (2 μg/mL) or BsAb (2 μg/mL) plus appropriate polyclonal anti-IgG antibody (20 μg/mL), and analyzed immediately using a FACS Vantage. Shown are plots of FL5/ FL4 versus time. An increase in the ratio of FL5/FL4 indicates an increase in the level of intracellular free calcium. BsAb specificity is as indicated. Control BsAb was the non-binding [huCD64 × A31 Id]. Similar results were obtained in at least 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/10/10.1182_blood.v96.10.3544/5/h82200347009.jpeg?Expires=1735835114&Signature=zcvrjKzMXLSgpvHjVZ-eE7ogorPyN~84V8JHiNNcyNDvdasiWKW7A-nxD9rPYr5gWH9gIoBEgnarET6LMD9rHAqLS8yjeRTZHnjLl0jl4CdkwdPFBT18UE37~sQDOVpra8eqjzO~O6WJxK~jHGLuMQ9pgKkSSucyeOQgKXYe75jT1Xvclrwhw1e-lhzZuyZLJaQqVQjuEppUWU8mXYc~2qtduZw3SjT0BuVaMY7o5ggEkmX~76SFXcEoPg0lAKXz7xvkOzLwseFrjbrLjkhnjoFWn9nDoMqbz80Ia6sth6eUR~uAsBYzoMhEzZnSKYfxFVnD0tFuClOv2UkUH5w0gA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Measurement of the levels of intracellular free calcium in lymphoma cells treated with BsAb.
πBCL1 were loaded with Indo-1-am fluorescent dye, warmed to 37°C for 5 minutes, and then incubated with either BsAb alone (2 μg/mL) or BsAb (2 μg/mL) plus appropriate polyclonal anti-IgG antibody (20 μg/mL), and analyzed immediately using a FACS Vantage. Shown are plots of FL5/ FL4 versus time. An increase in the ratio of FL5/FL4 indicates an increase in the level of intracellular free calcium. BsAb specificity is as indicated. Control BsAb was the non-binding [huCD64 × A31 Id]. Similar results were obtained in at least 3 separate experiments.
