Non-colocalization of HPTS-SL with various cellular compartments in DC.

Human DC were incubated with HPTS-SL for 24 hours. After incubation, SL were washed out, and cells were mounted on slides and stained intracellularly (for details, see “Materials and methods”) with control IgG or antibodies against LAMP-1, the macrophage mannose receptor (MMR), MHC class II (HLA-DR), and the transferrin receptor (TfR). Results are shown as HPTS-SL only (excitation for green, top), antibody staining (excitation for red, middle), and the computerized overlay of these pictures (bottom). Yellow in the bottom panels indicates colocalization of green and red (magnification, 1000×).