Fig. 3.
CD4+ T-cell proliferation induced by presentation of SL-encapsulated tetanus toxoid.
Mature human DC were pulsed with either soluble (sTT) or encapsulated tetanus toxoid (TT-SL) at 2.0 μg/mL for 24 hours. DC cultured in medium alone (med.) or pulsed with empty SL (SL, based on lipid concentration) were included as controls. Antigen was washed out, and DC were cocultured with 1 × 105 syngeneic T cells in a ratio of 1:10 (dark gray bars) or 1:30 (light gray bars). T-cell proliferation was monitored by measuring the uptake of thymidine, and mean cpm × 10−3 ± SEM of triplicate cultures from a representative experiment are shown.
