Fig. 4.
Presentation of graded doses of sTT or TT-SL by DC at different DC:T-cell ratios.
Mature (A, C) and immature (B, D) human DC were generated and added in a ratio of 1:10 (dark gray bars) or 1:30 (light gray bars) to 1 × 105 syngeneic T cells. Antigens (sTT or TT-SL) were directly added to the wells at 2.0 (A, B) or 0.02 (C, D) μg/mL. DC:T cell cocultures without antigen (med.) or pulsed with empty SL (SL, based on lipid concentration) were controls. T-cell proliferation was assessed by measuring the uptake of thymidine (as in Figure 3).
