American Society of Hematology

Fig. 8.

$Fig. 8. DC are the APCs involved in presentation of SL-encapsulated antigen in vivo. / Two mice were injected with 10 μg OVA-SL subcutaneously. Draining LN (dLN) and nondraining LN (ndLN) were removed 3 days later. Single-cell suspensions from LN were pooled, separated magnetically into CD11c+ and CD11c− fractions, and applied in graded doses to 3 × 105 CD8+ T cells from OT-1 mice. After 24 hours, T-cell proliferation was assayed by thymidine incorporation. Mean cpm × 10−3 from a representative experiment (1 of 2) are shown.$

DC are the APCs involved in presentation of SL-encapsulated antigen in vivo.

Two mice were injected with 10 μg OVA-SL subcutaneously. Draining LN (dLN) and nondraining LN (ndLN) were removed 3 days later. Single-cell suspensions from LN were pooled, separated magnetically into CD11c⁺ and CD11c⁻ fractions, and applied in graded doses to 3 × 10⁵ CD8⁺ T cells from OT-1 mice. After 24 hours, T-cell proliferation was assayed by thymidine incorporation. Mean cpm × 10⁻³ from a representative experiment (1 of 2) are shown.

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