Fig. 3.
![Fig. 3. Detection of [32P]lysophospholipids in [32P]orthophosphate-labeled platelets. / Human platelets labeled with [32P]orthophosphate were incubated without (−) or with 1 U/mL of thrombin (Thr), 20 μg/mL of collagen (Col), or 5, 10, or 20 μmol/L Sph for 5 minutes. Sph-1-P and LPA were first extracted from these platelets into the upper aqueous phase under alkaline conditions, followed by re-extraction into the lower chloroform phase under acidic conditions. The final chloroform phase was analyzed by TLC autoradiography.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/10/10.1182_blood.v96.10.3431/5/h82200364003.jpeg?Expires=1735829978&Signature=YAwahghTLrXXLMQlUL1qLRUknKg5Cmiz8MnHW0~Q6oiqXcD8cmRrUjvK~amuPX2leRiMzpGDGHrH-FmXf4PPerKM~kXkbQiIpmq7s1VvltKk1AlFFwpHtR0uHBkPQoIlryjZlZfXkFqL5yUpOprH9OVaOJIrgG~mvKjbI6VP-HNfJbbwueCkNlZCZi2oLQyw-xtfEFO0OgHyaCeiWlb8bDLqC6DU4V~u-2swSfimV4WlESX1tcH7u4c7KFb~5FIKOgO6Ms4bHJGipIGVPoTy2SQ7TGATJ4bhIjEryrCXYAnT8EgCeNmS8dDHyLKWhPCgAXGKlbdkV5FrsjwK9rONEQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Detection of [32P]lysophospholipids in [32P]orthophosphate-labeled platelets.
Human platelets labeled with [32P]orthophosphate were incubated without (−) or with 1 U/mL of thrombin (Thr), 20 μg/mL of collagen (Col), or 5, 10, or 20 μmol/L Sph for 5 minutes. Sph-1-P and LPA were first extracted from these platelets into the upper aqueous phase under alkaline conditions, followed by re-extraction into the lower chloroform phase under acidic conditions. The final chloroform phase was analyzed by TLC autoradiography.
