Fig. 4.
![Fig. 4. Formation of [32P]PA in [32P]orthophosphate-labeled platelets stimulated with thrombin or collagen. / Platelets labeled with [32P]orthophosphate were incubated without (−) or with 1 U/mL of thrombin (Thr), 20 μg/mL of collagen (Col), or 5 μmol/L Sph for 5 minutes. The lipids were extracted from these platelets, and the lower chloroform phase under alkaline conditions was analyzed for [32P]PA formation.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/10/10.1182_blood.v96.10.3431/5/h82200364004.jpeg?Expires=1735508275&Signature=ZSKxNqXYijb4jAywXaGHND9hVRwr96zR37asBn3g1ev~45~2i9NuFWso9APH8I-IZbd~8lhAQ5uwdKEiLPERiCV4xU8-quKL4CchmaeT5YE3BwS45lcCeh3a3lWyiXftevf6A5RVf7sq~X6fkJ3J9EkCoAWnGwLBB0af5nI5qadVAxFR~O0pZgV5bmZCgMWsPh-kvISgzpcrhMBG6Fsasa5PF0QtTrQtK1tZKlx6S6OWgqD-EsI4hbQNZMTLqgFuoME7xPJ~5yQ-zbN2DLOdbg36PQgm9WPKg55An6PtTkChY0ikOz6-L0-qOpJW1U4GZXYlUQWK7ag41HjLN-xlSQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Formation of [32P]PA in [32P]orthophosphate-labeled platelets stimulated with thrombin or collagen.
Platelets labeled with [32P]orthophosphate were incubated without (−) or with 1 U/mL of thrombin (Thr), 20 μg/mL of collagen (Col), or 5 μmol/L Sph for 5 minutes. The lipids were extracted from these platelets, and the lower chloroform phase under alkaline conditions was analyzed for [32P]PA formation.
