Fig. 6.
Co-fractionation of [3H]Sph-1-P with BSA.
Sph-1-P, containing trace amounts of [3H]Sph-1-P, was incubated with equimolar amounts of intact BSA (A) or denatured BSA (B). The mixtures were fractionated in a gel-filtration column, and fractions were collected and assayed for their [3H]Sph-1-P radioactivity and BSA concentration.