Interaction of endogenous PLZF and AML-1/ETO in a t(8;21) M2 leukemia cell line.

SKNO-1 cells were lysed and subjected to immunoprecipitation–immunoblot analysis. (A) Immunoblot with PLZF mouse monoclonal antibody. Lane 1, direct immunoblot of SKNO-1 cell lysate. Lane 2, blot of immunopellet. Lane 3, lysate supernatant after immunoprecipitation. Lanes 4-8, extracts immunoprecipitated with mouse control antibody, rabbit control antibody, AML-1 antibody, ETO antibody, and PLZF antibody. (B) Immunoblot with AML-1 rabbit polyclonal antibody. Lane 1, direct immunoblot of SKNO-1 cell lysate. Lane 2, blot of immunopellet. Lane 3, lysate supernatant after immunoprecipitation. Lanes 4-8, extracts immunoprecipitated with mouse control antibody, rabbit control antibody, AML-1 antibody, PLZF antibody, and ETO antibody. (C) Immunoblot with ETO rabbit polyclonal antibody. Lane 1, direct immunoblot of SKNO-1 cell lysate. Lane 2, lysate supernatant after immunopelleting. Lanes 3-7, extracts immunoprecipitated with mouse control antibody, rabbit control antibody, AML-1 antibody, PLZF antibody, and ETO antibody.