AML-1/ETO antagonizes the transcriptional repression mediated by both repression domains of PLZF.

293T cells were transfected with the (GAL4)₅-tk-Luc reporter. (A) Lanes 1 to 4 (12-well dishes), 400 ng GAL4^1–147, GAL4-PLZF, and AML-1/ETO as indicated. Results are expressed as fold repression compared to vector. (B) Transfections identical to those in A, with results shown as relative luciferase levels. (C) Lanes 1 to 4 (12-well dishes), 400 ng GAL4^1–147, GAL4-BTB/POZ, and AML-1/ETO as indicated. Lanes 5 to 14 (6-well dishes), 800 ng GAL4^1–147or GAL4-BTB/POZ was cotransfected with increasing doses of AML-1/ETO as follows: lanes 6 and 11, 100 ng; lanes 7 and 12, 200 ng; lanes 8 and 13, 400 ng; lanes 9 and 14, 800 ng, respectively. (D) Transfections with GAL4-RD2 with identical quantities as in the experiments described in C. (C,D) Fold repression compared to vector only. The results of all graphs reflect the average of multiple experiments ± SEM.