Clonal growth of 32D cells in methylcellulose.

(A) Transfected 32Dcl3 cells were plated at a concentration of 300 cells per dish. Colonies were counted on day 6. The assays were plated as quadruplicates. The numbers given show the results of one of at least 3 independent experiments per construct, which all gave similar results. IL-3; 1 ng/mL, FL; 20 ng/mL. (B) Transfected cells were plated at 3000 cells per dish under the indicated growth conditions. Photographs of the dishes were taken on day 6. IL-3, 1 ng/mL; FL, 200 ng/mL.