Tie-2 expression on KSLA fetal liver cells.

After panning with AA4.1 antibody, the cells were stained with FITC–anti-lineage markers, PE–anti-Sca-1, APC–anti-Kit, and biotinylated anti–Tie-2, followed by streptavidin-Texas Red. (A) The gate for lineage-negative or -low (lin^−/low) cells upon FACS analysis. Lin^−/low cells consisted of 37.4% ± 2.1% (mean ± SE, n = 8) of AA4.1-enriched cells. (B) Kit-positive, Sca-1–positive cells among lin^−/lowAA4.1⁺ cells. (C) The sorting gates for Tie-2–positive (T⁺ KSLA) and Tie-2–negative (T⁻ KSLA) cells.