Colony formation of KSLA, T+ KSLA, and T− KSLA cells.
A single-cell culture of a total of 240 cells was performed for each KSLA, T+ KSLA, and T− KSLA cell population. C-, L-, M-, and H-level cells formed colonies consisting of fewer than 50, 50 to 100, 100 to 104, and more than 104cells, respectively. C-level cells represented 25.8% ± 12.1%, 26.7% ± 11.6%, and 25.8% ± 3.6% (mean ± SD, n = 3) of KSLA, T+ KSLA, and T− KSLA cells, respectively. L-level cells represented 3.6% ± 0.5%, 1.7% ± 1.7%, and 1.7% ± 1.7% of KSLA, T+ KSLA, and T− KSLA cells. M-level cells represented 15.8% ± 2.2%, 35.6% ± 5.9%, and 9.7% ± 2.7% of KSLA, T+ KSLA, and T− KSLA cells. H-level cells represented 4.4% ± 1.9%, 15.3% ± 4.9%, and 1.9% ± 2.1% of KSLA, T+ KSLA, and T− KSLA cells.
