Reconstitution analysis of the adult and newborn recipients.
Ten T+ KSLA cells (B6-F1) and 2 × 105 bone marrow cells (B6-Ly5.1) were mixed and transplanted into either irradiated adult or conditioned newborn mice. Twelve weeks after transplantation, peripheral blood cells of the recipients were analyzed on a FACS. The representative results for adult (A) and newborn (B) recipients are shown. F1-type cells, presenting both Ly5.1 and Ly5.2 antigens, were derived from T+ KSLA cells. Ly5.1-type cells were derived from bone marrow competitor cells. Note the large number of host-derived cells (Ly5.2 type) in the case of a newborn recipient (Bi). Myeloid (Aii and Bii), B-lymphoid (Aiii and Biii), and T-lymphoid (Aiv and Biv) cells are present within the gate of fetal liver–derived cells (F1 type).
