Fig. 1.
Log linear relationship between measured and actual copy number of EBV genomes.
The measured copy number of EBV genomes determined by EBV QC-PCR were log linear with the actual copy number of EBV genomes. Known number of Namalwa cells (containing 2 copies of EBV genomes per cell) were mixed with EBV− CA46 cells for a total of 106 cells per aliquot. Each aliquot was spiked with 200 copies of ICS before DNA isolation and QC-PCR. Each data point represents the mean and standard deviation of 4 to 7 separate experiments over a period of 1 year. The curve was fitted by linear regression analysis and the equation for the curve is log(added-EBV) = 1.58 × log(measured-EBV) − 1.71.
