Fig. 2.
Copy number of EBV genomes in peripheral blood of healthy carriers, patients with PTLD in remission, and patients with active PTLD.
DNA from 1 × 106 PBMCs spiked with 200 copies of ICS was isolated and eluted into 100 μL dH2O. For QC-PCR, 40 μL DNA was used and the EBV load measured and calculated as described in Figure 1. EBV loads in healthy carriers are the average of 6 separate experiments. DNA was isolated independently for each of these experiments.
