GFP gene expression in human CD45⁺ cells from a representative primary and corresponding secondary transplant recipient mouse.

The primary recipient (mouse 5.3) received a transplantation dose of 400 000 CD34⁺ lentivirally transduced cells. Six weeks after transplantation, bone marrow was harvested and 12 million mononuclear cells were transplanted into a secondary transplant recipient (mouse 5.3.3). A decrease in engraftment from primary to secondary recipients is seen but a high proportion of GFP⁺ cells are detected in this secondary recipient by FACS.