Lineage analysis of a transplant recipient with practically all human cells transduced.

A unique mouse receiving a low cell transplantation dose showing high-level transduction efficiency as determined by FACS for GFP. This mouse (7.23) received 35 000 lentivirally transduced CD34⁺cells. Six weeks after transplantation, bone marrow was harvested and analyzed for percent GFP⁺ human CD45⁺ cells. High-level transduction efficiency had been achieved with 91% of human cells expressing GFP. Lineage analysis for lymphoid (CD19) and myeloid (CD15/33) cells showed similar high-level transduction efficiencies in both lineages (ie, 91% expressing GFP in the lymphoid lineage and 93% in the myeloid lineage). All the human CFU-GM colonies derived from this mouse expressed the transgene.