Fig. 5.
IFN-α prevents spontaneous apoptosis of the clonal Th2 cells in a dose-dependent manner.
CD3−CD4+ cells from hypereosinophilic patient 1 were cultured in the presence of graded amounts of IFN-α (1-104 IU/mL) for 24 hours. At the end of the culture, apoptotic lymphocytes were detected by flow cytometry according to their light scatter properties (FSC/SSC). Results are the means ± SEM of 4 experiments. *P < .005, **P < .05 as compared with culture without IFN-α (ANOVA test).
