Functional responses of control and kinase-deficient platelets.

(A) Platelet shape change and aggregation stimulated with CRP or thrombin. (i) Wild-type and fyn^−/−platelets stimulated with CRP (0.3-3.0 μg/mL). (ii) Wild-type andfyn^−/−platelets stimulated with thrombin (0.1 U/mL). Results are representative of 5 experiments. (B) Fibrinogen binding and P-selectin exposure in fyn^−/−platelets stimulated by CRP in the presence of 1mmol/L CaCl₂. The results are mean ± standard error of mean of 4 experiments. ▪ indicates fyn^+/+; ■,fyn^−/−; *, P < .05; **,P < .01). (C) P-selectin exposure inlyn^−/− platelets stimulated by CRP in the presence of 1 mmol/L CaCl₂ or 1 mmol/L EGTA. The results are mean ± standard error of mean of 4 experiments. ▪ indicateslyn^+/+; ■, lyn^−/−; *,P < .05; **, P < .01.